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Product Introduction

Bioactivity

Description	Sodium Nitroprusside (Ro 21-2498) is a potent vasodilator, releasing in blood with no spontaneously.
In vitro	Administering 5 mg/kg of sodium nitroprusside, acting as a nitric oxide donor, effectively mitigates intestinal ischemia-reperfusion injury in rats.
In vivo	Sodium nitroprusside inhibits the proliferation of vascular smooth muscle. As an effective vasodilator, it exhibits pronounced dilation effects particularly in the arterioles and venules. During circulation, sodium nitroprusside decomposes to release nitric oxide (NO), which activates guanylate cyclase in vascular smooth muscle and augments intracellular cyclic guanosine monophosphate (cGMP) production, promoting the relaxation of the smooth muscle and consequent vasodilation.
Alias	Sodium Nitroferricyanide, Ro 21-2498, Nitroprusside Sodium

Chemical Properties

Molecular Weight	261.92
Formula	Na₂Fe(CN)5NO
Cas No.	14402-89-2

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 55 mg/mL (209.99 mM)

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.8180 mL	19.0898 mL	38.1796 mL	190.8980 mL
5 mM	0.7636 mL	3.8180 mL	7.6359 mL	38.1796 mL
10 mM	0.3818 mL	1.9090 mL	3.8180 mL	19.0898 mL
20 mM	0.1909 mL	0.9545 mL	1.9090 mL	9.5449 mL
50 mM	0.0764 mL	0.3818 mL	0.7636 mL	3.8180 mL
100 mM	0.0382 mL	0.1909 mL	0.3818 mL	1.9090 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

InhibitorinhibitSodium Nitroprusside